Quantitative PCR (qPCR) instruments that operate in real-time need high sensitivity and the most favorable signal-to-noise ratio possible. The bandwidth of excitation, center wavelength (CWL) and ...

In its formative years PCR and its intellectual possessors had to go through some legal wrangling over the originality of the technique. In point of fact, a 13-year earlier paper by Nobelist Gobind ...

First, the BAC clone DNA is denatured into single stranded templates. Each of the three DOP-PCR primers used in the next step is flanked by known 5' and 3' anchor sequences with six random nucleotides ...

Researchers have developed a plasmofluidic chip that can perform PCR in only about 8 minutes, which could speed diagnosis during current and future pandemics. Reverse transcription-polymerase chain ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...

Since its invention in the 1980s, polymerase chain reaction (PCR) opened the door for applications such as genetic engineering, genotyping, and sequencing, and has become a fundamental part of ...

Polymerase chain reaction (PCR) tests are one of the most popular diagnostic tools for the detection and diagnosis of viral diseases, such as coronavirus (COVID-19), due to their ability to rapidly ...

A new discovery has reshaped our understanding of PCR chip technology: water vapor, not air expansion, is the primary culprit behind bubble formation in polydimethylsiloxane (PDMS)-based chips. This ...

Polymerase chain reaction (PCR) test kits for COVID-19 use what’s known as gene cloning – not reproductive cloning – to detect the presence or absence of the SARS-CoV-2 virus, but social media posts ...

Researchers from the Chinese Academy of Sciences’ Shanghai Institute of Microsystem and Information Technology published a new high-pressure liquid seal method in Microsystems & Nanoengineering on ...